windows安装aspera高速下载sra文件/fastq格式

安装aspera并配置
根据srr号在ena网站找链接并下载
笔记·：
先去NCBI找项目编号SRPxxx
每个项目都有许多生物样本，SAMN或SRS编号
对样本做多次实验，用SRX编号区分每次实验
一次实验会有很多数据，SRR（或ERR、DRR）文件
一般是下载对应项目的所有SRR文件
或者是对应实验的所有SRR

SRR文件原始格式是sra格式
要拿去做分析一般要转成fastq格式

然后去ENA搜索项目编号SRP，可以看到有很多原始数据就是SRRxxx，选择要下载的内容，下载tsv文件
在tsv文件找到下载链接

powershell输入命令下载
举个例子

本来我拿到的是fasp.sra.ebi.ac.uk:/vol1/fastq/SRR290/017/SRR29039017/SRR29039017_1.fastq.gz
fasp.sra.ebi.ac.uk:/vol1/fastq/SRR290/017/SRR29039017/SRR29039017_2.fastq.gz
输入命令

ascp-QT-l300m-P33001-i"C:\Program Files\IBM\Aspera Connect\etc\asperaweb_id_dsa.openssh"era-fasp@ fasp.sra.ebi.ac.uk:/vol1/fastq/SRR290/017/SRR29039017/.

需要更改的地方：
asperaweb_id_dsa.openssh文件的地址："C:\Program Files\IBM\Aspera Connect\etc\asperaweb_id_dsa.openssh"改成自己的

era-fasp@后面写找到的链接

我这个命令下载SRR29039017文件夹了，就是那两个文件

多文件下载没用过，教程有

Windows PowerShell 版权所有（C） Microsoft Corporation。保留所有权利。 安装最新的 PowerShell，了解新功能和改进！https://aka.ms/PSWindows 加载个人及系统配置文件用了1676毫秒。(base)PS C:\Users\Lenovo>ascp-hUsage: ascp.exe[OPTION]SRC... DEST SRC to DEST, or multiple SRC to DESTdirSRC, DEST format:[[user@]host:]PATH-h,--help Display usage -A,--version Display version.-TDisable encryption-dCreate target directory, impliedforfile/file-pair lists-pPreservefiletimestamp-qDisable progress display-vVerbose mode-6Use IPv6 -D... Debug level-lMAX-RATE Max transfer rate-mMIN-RATE Min transfer rate RATE: G/g(gig),M/m(meg),K/k(kilo)-uUSER-STRING User-specific string-iPRIVATE-KEY-FILE Private-keyfilename(id_rsa)-wDIRECTION Test bandwidth. DIRECTION: r,f-KPROBE-RATE Bandwidth measurement, probes/sec-kRESUME-LEVEL Resume criterion:0,3,2,1-ZDATAGRAM-SIZE ManuallysetMTU-gREAD-SIZE File read-block size-GWRITE-SIZE File write-block size SIZE: K(kilo), M(meg), or just bytes-LLOCAL-LOG-DIR Local logging directory path-RREMOTE-LOG-DIR Remote logging directory path-SREMOTE-ASCP Name of remote ascpcommandline-OFASP-PORT UDP port usedforFASP transport-PSSH-PORT TCP port usedforSSH authentication-CM-ID:N-COUNT Multi-session transfer, move only Mth of N parts-NPATTERN Inclusion pattern. Repeatformore.-EPATTERN Exclusion pattern. Repeatformore.-fCONFIG-FILE Configuration-file name-WTOKEN-STRING Specify TOKEN-STRINGfortransfer -@ RANGE-LOW:RANGE-HIGH Transfer only a range of bytes withinfile-XREXMSG-SIZE Size of retransmit request-cCIPHER File data cipher: aes128, aes192, aes256, aes128cfb, aes192cfb, aes256cfb, aes128gcm, aes192gcm, aes256gcm--mode=MODE MODE: send, recv--user=USERNAME--host=HOSTNAME--policy=TRANSFER_POLICY TRANSFER_POLICY: fixed,high,fair,low --file-list=FILENAME File with list of sources --file-pair-list=FILENAME File with list of src/dest pairs --source-prefix=PREFIX Prepend to each SRC path --symbolic-links=METHOD METHOD: follow,copy,copy+force,skip --remove-after-transfer Remove SRC files after transfer success --remove-skipped Remove skipped SRC files after transfer --move-after-transfer=ARCHIVE Move SRC files under path ARCHIVE after transfer success --remove-empty-directories Remove empty SRC subdirectories --remove-empty-source-directory Also remove thesourcedirectory itselfifempty --save-before-overwrite Renamefileinstead of overwrite --file-manifest=OUTPUT OUTPUT: text,none --file-manifest-path=DIRECTORY --file-manifest-inprogress-suffix=SUFFIX --precalculate-job-size --precalculate-disable--overwrite=METHOD METHOD: never,always,older,diff,diff+older --file-crypt=CRYPT CRYPT: encrypt,decrypt --file-checksum=HASH HASH: sha-512,sha-384,sha-256,sha1,md5,none --partial-file-suffix=SUFFIX Default issetto""disabling the partialfileusage --src-base=NAME Preserve directory structure of SRC arguments below NAME --src-base64=NAME Preserve directory structure of SRC arguments below NAME(base64 encoded)--proxy=URL --allow-password-prompt Promptsforuser password entry onsshconnection --ignore-host-key Promptsforuser password entry onsshconnection(deprecated)--check-sshfp=FINGERPRINT Required server SSHhostkey fingerprint --apply-local-docroot Applylocaldocroot --preserve-xattrs=MODE MODE: native,metafile,none --remote-preserve-xattrs=MODE MODE: native,metafile,none --preserve-acls=MODE MODE: native,metafile,none --remote-preserve-acls=MODE MODE: native,metafile,none --output-file-progress Outputfileprogress wheninbackground mode or redirecting output to afileor pipe --multi-session-threshold=THRESHOLD Skip multi-sessioniffilesize islessthan threshold --delete-before-transfer Delete extraneous files from DEST before any files are transferred --preserve-object-lock-legal-hold Preserve legal hold status ofsourcefiles/directories when created at destination --preserve-object-lock-retention Preserve retention info ofsourcefiles/directories when created at destination --preserve-object-metadata Preserve object metadata HTTP fallback only options:-y0/11=Allow HTTP fallback(default=0)-IFILENAME HTTPS certificatefilename-tPORT HTTP fallback server port-xPROXYSERVER-ADDR[:PORT]Proxy address and port(default80)(base)PS C:\Users\Lenovo>d:(base)PS D:\># 创建并进入D盘的SRA_Data目录（没有就新建）(base)PS D:\>mkdirD:\SRA_Data 目录: D:\Mode LastWriteTime Length Name ---- ------------- ------ ---- d-----2026/3/139:49 SRA_Data(base)PS D:\>cdD:\SRA_Data(base)PS D:\SRA_Data>ascp-i"C:\Program Files\IBM\Aspera Connect\etc\asperaweb_id_dsa.openssh"-Lfasp@fasp.sra.ebi.ac.uk:/vol1/fastq/SRR290/017/SRR29039017/ ./ error initializing transfer log'fasp@fasp.sra.ebi.ac.uk:/vol1/fastq/SRR290/017/SRR29039017/aspera-scp-transfer.log'(AS_UNKNOWN_ERRNO). ascp.exe: Error initializing FASP(No suchfileor directory)Startup failed,exit(base)PS D:\SRA_Data>ascp-QT-l300m-P33001-i"C:\Program Files\IBM\Aspera Connect\etc\asperaweb_id_dsa.openssh"era-fasp@fasp.sra.ebi.ac.uk:/vol1/fastq/SRR290/017/SRR29039017/.SRR29039017_1.fastq.gz100% 1698MB25.7Mb/s 03:19 SRR29039017_2.fastq.gz100% 1704MB40.0Mb/s 08:08 Completed: 3483915K bytes transferredin488seconds(58381K bits/sec),in2files,1directory.(base)PS D:\SRA_Data>